Diemerbroeck studied at Utrecht before moving to Angers in France, where he received his M.D. in 1634. He returned to Nijmegen where a plague epidemic broke out in 1635. His account of the plague, De peste was reprinted many times. In 1649 he was appointed professor of anatomy and medicine at the University of Utrecht. His highly popular Anatome corporis humani, was first published at Utrecht in 1672, and reprinted at Lyon in 1676, 1679, and 1683, and at Geneva in 1679. This English translation was done by William Salmon.